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Image Search Results
Journal: Gynecologic oncology
Article Title: Panobinostat sensitizes cyclin E high, homologous recombination-proficient ovarian cancer to olaparib
doi: 10.1016/j.ygyno.2016.07.088
Figure Lengend Snippet: A) Raw RSEM RNA-Seq counts for 265 TCGA ovarian tumors. CCNE1 (cyclin E) expression was compared to E2F1, BRCA1 and RAD51 (Spearman correlation). B) CCNE1 copy number in CCLE ovarian cancer cell lines. C) Spearman correlation of cyclin E and E2F1 mRNA levels in CCLE cell lines most representative of serous ovarian cancer (similarity score >1) based on ref [29]. D) Western blot analysis of cyclin E and E2F1 expression in representative CCLE cell lines. Actin was the loading control.
Article Snippet: Antibodies used were
Techniques: RNA Sequencing Assay, Expressing, Western Blot
Journal: Gynecologic oncology
Article Title: Panobinostat sensitizes cyclin E high, homologous recombination-proficient ovarian cancer to olaparib
doi: 10.1016/j.ygyno.2016.07.088
Figure Lengend Snippet: A) Concentration-dependent effects of panobinostat and vorinostat in SRB viability assays in OVCAR-3 cells (72h). Values are mean+SE of 3 independent experiments. B) Western blot analysis of the effects of panobinostat (25nM), vorinostat (5µM) and romidepsin (10nM) on expression of cyclin E1, E2F1, BRCA1, cleaved PARP and acetylated histone H3 in OVCAR-3 cells. Actin and histone H3 were loading controls. C) Effects of the panobinostat pre-treatment (25nM; 24h)/panobinostat (25nM; 24h) and olaparib (10µM) co-treatment combination regimen (24h) on cyclin E, E2F1 and BRCA1 expression in OVCAR-3 cells (24h). Actin was the loading control.
Article Snippet: Antibodies used were
Techniques: Concentration Assay, Western Blot, Expressing
Journal: Gynecologic oncology
Article Title: Panobinostat sensitizes cyclin E high, homologous recombination-proficient ovarian cancer to olaparib
doi: 10.1016/j.ygyno.2016.07.088
Figure Lengend Snippet: A) Schematic of drug treatment experiment in nude mice injected subcutaneously with SKOV-3 cells. Mice (10 per group) were pre-treated for one week with vehicle or panobinostat, and then subsequently with vehicle, panobinostat and/or olaparib for three weeks as shown (IP: intraperitoneal; PO: per os, oral gavage). B) Time course measurements of tumor volume at weekly intervals (single arrow: start of panobinostat pre-treatment; double arrow: start of full drug regimen). C) Tumor weights at sacrifice. Tumors are shown in D). E) Western blot analysis of cyclin E, BRCA1, cleaved PARP, PCNA and pH2AX protein expression in harvested tumors. Actin and histone H3 were loading controls. F) Densitometry analysis of expression relative to corresponding actin or histone H3 levels. Values are mean+ SD; *p<0.01 single drug effect relative to vehicle; ap<0.01 combination drug effect relative to olaparib; bp<0.01 combination drug effect relative to panobinostat, Student’s t test.
Article Snippet: Antibodies used were
Techniques: Injection, Western Blot, Expressing
Journal: iScience
Article Title: The Y14-p53 regulatory circuit in megakaryocyte differentiation and thrombocytopenia
doi: 10.1016/j.isci.2021.103368
Figure Lengend Snippet:
Article Snippet:
Techniques: Virus, shRNA, Recombinant, Flow Cytometry, Plasmid Preparation, Mutagenesis, Expressing, Software